Annogen introduces AIM™ service

25 June 2022

Identify favorable gene insertions and their expression levels (> 100,000 integrations in parallel)

Annogen introduces a new service to identify gene insertions and asses their expression levels for >100,000 integrations in parallel. Using an adaptation of its proprietary SuRE™ barcoding strategy and previously established protocols, Annogen has now scaled a gene-insertion and expression mapping strategy to unprecedented numbers.

AIM™ applications

  • Genetic engineering T cells, NK cells, B cells
  • E.g. Expression upon T-cell activation or in specific lineage
  • Production lines for stable expression
  • E.g. CHO sites for maximum expression level
  • Production lines for induced expression
  • E.g. HEK293T sites for induced expression of Rep protein
  • Reporter lines for drug screens…
  • E.g. Fluorescence upon inflammation, proliferation, apoptosis, …

Example of AIM™ screen in Jurkat cells

Integrations Heatmap N=310807

Identify loci that are only active upon T-cell activation

Conclusion

300-fold induction upon Jurkat stimulation with lonomycin and PMA

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