A single base can make or break a regulatory element. With SuRE™, we measure the effect of every single-base change across a promoter or enhancer, resulting in a complete functional map rather than a prediction. That tells you which changes make your own promoter stronger, more specific or smaller, and which therapeutic base edits could retune an endogenous gene to cure disease.
Effects in non-coding regulatory elements are hard to read from sequence: one base can shift expression sharply, or have no effect at all. Whether you want to make your current promoter stronger, more specific or smaller, or find the base edits that would retune an endogenous gene for therapy, the question is the same: which changes actually move expression?
With SuRE™, we measure large numbers of variants in parallel, including every single-base change across a region by saturation mutagenesis, and quantify each one’s effect on regulatory activity. Annogen does not do the editing in the organism. We identify which changes are functionally relevant, and by how much, so your team moves downstream with a shortlist backed by measurement, not prediction.
Pinpoint which non-coding variants affect expression, for example, the causal variant among many candidates from a GWAS.
Identify which candidate base edits are most likely to create the desired change in regulatory activity.
Screen large numbers of variants in parallel to generate training or validation data for improved modelling.
What a project looks like depends on the organism, trait, and candidate sequence space. Typically, it involves these steps:
SuRE™ is Annogen’s platform for large-scale functional screening of regulatory sequences. Here, it’s used to compare candidate variants in parallel and measure their effect on regulatory activity, turning a long list of possible edits into a shorter, experimentally supported set.
Let’s start with your ambitions.
Share your challenge, question or how we could improve your therapy or crop. We will help you explore how tailored gene expression can unlock tomorrow’s innovations.
