Platform for discovering optimal expression loci

The genomic position where a therapeutic gene is integrated determines its stability and (conditional) expression levels. Understanding the positional effects of potential integration sites is therefore critical both in designing effective and safe cell therapies as well as in designing biologics manufacturing systems.

Annogen’s Integration Mapping (AIM™) technology barcodes >100,000 genomic loci to determine the most optimal sites for expression of a transgene. A barcoded reporter gene (e.g. GFP or your GOI), driven by a promoter (e.g. a minimal promoter), is stably integrated into >100,000 unique genomic sites, with each site being labelled with a unique barcode. Measuring barcoded RNA transcripts from each locus allow for sensitive interrogation of the position effect on expression levels, stability and cell type-specificity.

The AIM™ platform

The Annogen Integration Mapping (AIM) platform identifies genomic loci with desiredtranscriptional characteristics, e.g. stable, high expression levels. AIM™ can be applied to any cultured cell type of interest. 

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