Discover SuRE™
Platform for bespoke promoter engineering
Why promoters matter in achieving your goals
Promoters and enhancers are DNA-based switches naturally occurring in the genome, which determine when and where a gene is expressed. In each organism, millions of years of evolution have optimized these genomic elements to regulate gene expression. Finding and using the right switches is pivotal in making safe advanced therapies, optimizing biologics manufacturing and enabling trait discovery in crops.
Using its proprietary Survey of Regulatory Elements (SuRE™) platform, Annogen can find, characterize and optimize promoters and enhancers for your applications. The eventual identified hybrid elements are unique to your project and can be patented by you, providing you with additional IP protection for your therapy, crop or expression system.
The proof-of-concept paper for SuRE™ was published by Van Arensbergen et al. in Nature Biotechnology (link).
The eventual hybrid elements are unique to your project and can be patented by you, ensuring that you have exclusive freedom to operate on the most optimal promoter for your therapy, crop or expression system.
Experimental validations rather than predictions for promoter design
Unlike the most typical promoter identification tools that rely largely on unvalidated (e.g. machine learning-based) predictions, SuRE™ delivers the power of millions of real-world, wet-lab measurements in relevant cells and in vivo models, providing validated elements that support more effective and safer therapies.
At Annogen, we have experience developing promoters for many organisms, tissue types and (stimulus) conditions. The SuRE™ workflow can be tailored to a client’s requirements in terms of e.g. element size, expression levels and on-target and off-target cells types.
Collaborative study design
Annogen’s screening projects are always designed in close collaboration with clients to ensure that we develop the right genetic engineering system for you. The discussions can include the elements to be tested and which on-target and off-target in vitro or primary cell types are assayed. Annogen has instant access to dozens of cell lines, while clients can also provide their own cell lines if desired.
Bayer, BASF, Orchard Therapeutics, Novo Nordisk, uniQure, Meira GTx, VectorY, Pfizer and dozens of other companies have already trusted Annogen to perform SuRE™ analyses for their projects.
Annogen’s SuRE™ promoter screening platform setup
After each work package, Annogen involves its clients in the selection of regulatory elements to be included in subsequent work packages. The client has the option to become to exclusive owner of the generated promoters.
New! Performing SuRE in vivo
Besides performing in vitro SuRE™ from a plasmid based system, SuRE™ screens can also be performed using a lentiviral (Lenti-SuRE™) or AAV (AAV-SuRE™) vector, allowing one to perform an unbiased parallel screen in animals to better understand the in vivo performance of regulatory elements. Annogen has experience with testing >10,000 promoters in vivo in parallel.
Broad applications
In addition to human applications, SuRE™ can be applied to any organism, with applications ranging from agricultural crops to stable protein production cell lines. This often involves the generation of SuRE™ libraries with tailored genomes or pre-selected sequences.
Cell type-specific promoters
Ideal for targeting specific cells, such as neural or cardiac cells in gene therapy.
Stimulation-specific promoters
Designed to respond to specific conditions, such as neuroinflammation or T-cell activation.
Stable and constitutive promoters
Perfect for applications requiring consistent gene expression, like CHO promoters for recombinant protein production.
Learn how SuRE™ can optimize the expression of your transgene
Related services
Cell & gene therapies
Manufacturing cell lines
Crop engineering
“To me, imagining the possibilities of SuRE™ applications feels like walking in a candy store.”
Dr. Martijn Kelder, Chief Strategy Officer
