Identify favorable gene insertions and their expression levels (> 100,000 integrations in parallel)
Annogen introduces a new service to identify gene insertions and asses their expression levels for >100,000 integrations in parallel. Using an adaptation of its proprietary SuRE™ barcoding strategy and previously established protocols, Annogen has now scaled a gene-insertion and expression mapping strategy to unprecedented numbers.
AIM™ applications
- Genetic engineering T cells, NK cells, B cells
- E.g. Expression upon T-cell activation or in specific lineage
- Production lines for stable expression
- E.g. CHO sites for maximum expression level
- Production lines for induced expression
- E.g. HEK293T sites for induced expression of Rep protein
- Reporter lines for drug screens…
- E.g. Fluorescence upon inflammation, proliferation, apoptosis, …
Example of AIM™ screen in Jurkat cells
Integrations Heatmap N=310807
Identify loci that are only active upon T-cell activation
Conclusion
300-fold induction upon Jurkat stimulation with lonomycin and PMA
